FastQCFastQC Report
Mon 27 Feb 2023
SRR3127158.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127158.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1311767
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149281.1380069783734459No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC65670.5006224428576111No Hit
GCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTG45240.34487832061638996Illumina PCR Primer Index 5 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTG38770.2955555369208099Illumina PCR Primer Index 5 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC36540.27855556665169956Illumina PCR Primer Index 5 (95% over 23bp)
AAAGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTT23240.17716560944131082No Hit
AGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCT14720.11221505038623476Illumina PCR Primer Index 5 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACGC254.920166E-794.004577
ACGGGAT5100.085.710054
CGTTTTT52100.085.192681
ATAGCGG2150.083.102321
GCTACGA11050.080.393054
CGAAGGG10900.080.196572
AGGGATG31800.079.815215
CGTAGGG2250.079.372572
AAGGGTA9750.079.0602654
CGCTACG11350.078.682243
CGGGTAT1800.078.337145
GGATGGC21300.078.116487
TAGCGGG4900.077.688782
CTACGAC11550.076.912835
TCCGCTA11700.076.35471
AGAGGGA32850.075.547063
TCGGGTA507.094059E-1175.203664
AGGGTAT7850.074.8444065
TACGACC11900.074.2557146
AAGAGGG40800.073.835842
Produced by FastQC (version 0.11.5)