FastQCFastQC Report
Mon 27 Feb 2023
SRR3127155.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127155.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1543440
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185571.20231431088996No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT49870.3231094179235992No Hit
GCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTG43700.28313377909086196No Hit
CTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGC39870.25831907945887106No Hit
CCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTG39080.2532006427201576No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16200.10496034831285958No Hit
AGCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCT16130.1045068159436065No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT52350.083.6983641
ACGGGTA1850.081.298674
GGTAAGG7850.079.053671
GGATAGG8150.074.990031
ATAGGGC8750.074.664123
AGACGGG11000.072.635232
GAATAGG7450.072.570371
AGTAGGG21700.070.390612
TAGCGGG8300.070.2157752
GCATAGG4100.069.9461441
CTATAGG1550.069.76121
TAGGGCA6950.069.655854
ATAGCGG3250.069.4345551
TAGGGAC7550.069.100514
AGGGCAT11350.068.739025
TATAGGG10000.068.6189352
ATAAGGG19250.068.1184162
CGAAGGG14700.068.100982
GATAGGG21600.067.670252
AGGGACT35250.067.465625
Produced by FastQC (version 0.11.5)