FastQCFastQC Report
Mon 27 Feb 2023
SRR3127152.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127152.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences409630
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58631.4312916534433513No Hit
GCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTG21360.5214461831408832RNA PCR Primer, Index 14 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTG17590.42941190830749704RNA PCR Primer, Index 16 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGC15790.38546981422259113RNA PCR Primer, Index 16 (95% over 23bp)
AGCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCT6600.16112101164465492RNA PCR Primer, Index 16 (95% over 21bp)
AGAGCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTT5360.1308497912750531No Hit
CGCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCT5130.1252349681419818RNA PCR Primer, Index 16 (95% over 21bp)
AAAGCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTT5090.12425847716231722No Hit
GGCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCT4990.12181724971315577RNA PCR Primer, Index 16 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTT4960.12108488147840733No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT4820.11766716304958133No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG4800.11717891755974905No Hit
ACTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTG4550.11107584893684545RNA PCR Primer, Index 14 (95% over 22bp)
GATCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTC4300.10497278031394186No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTAGG301.3205863E-894.0322341
AGTACGG156.8896165E-494.0322341
CGGGTAT254.9161827E-793.99785
CACGGGC301.3240424E-893.99783
AACGGTC201.836691E-593.99783
ATATCGG156.899655E-493.99789
GCGTAGG900.088.808221
CGTAGGG1450.087.51522
CGTTTTT18200.084.990681
ACGGGTC452.7284841E-1183.5536044
TAGGGCA1350.083.5536044
ATAGGGC2050.082.534653
TAGACGG353.8491635E-880.599061
TACGAGG353.8491635E-880.599061
GAGTAGG2000.079.927411
CGAAGGG3000.079.898132
AGGGCAT3250.079.53665
AGGGTAC1200.078.3315055
AAGGGAC3400.077.409964
AGGGCAC1950.077.12645
Produced by FastQC (version 0.11.5)