FastQCFastQC Report
Mon 27 Feb 2023
SRR3127151.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127151.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences340484
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40571.1915391031590326No Hit
GCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTG19840.5826999212885187RNA PCR Primer, Index 14 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTG15680.46052090553447445RNA PCR Primer, Index 16 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGC14120.4147037746267079RNA PCR Primer, Index 16 (95% over 23bp)
AGCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCT5740.16858354577601295RNA PCR Primer, Index 16 (95% over 21bp)
GGCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCT5550.16300325419109268RNA PCR Primer, Index 16 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCT5270.15477966659226278RNA PCR Primer, Index 16 (95% over 21bp)
AGAGCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTT4970.14596867987923073No Hit
GATCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTC4690.13774509228040085No Hit
GAATCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTT4340.12746560778186347No Hit
AAAGCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTT4250.12482231176795386No Hit
ACTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTG4210.12364751353954959RNA PCR Primer, Index 14 (95% over 22bp)
GAAGCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTT3850.11307432948391115No Hit
ACCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCT3590.10543814099928338RNA PCR Primer, Index 14 (95% over 21bp)
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT3480.10220744587117161No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCC3420.10044524852856522No Hit
GCCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCT3410.10015154897146415RNA PCR Primer, Index 14 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACGG201.8104574E-594.265871
AGTACGG301.2973032E-894.265871
GTACGGG254.8487163E-794.2103652
TCGGGTA156.8369776E-494.210364
TACGGGT156.8369776E-494.210363
GGTATAT201.8370993E-593.988987
GGCCTAA156.901221E-493.988987
CGTTTTT13500.087.981481
CGAGGGT750.087.9296653
GTAGCGG700.087.532591
GCACGGG650.086.963422
ATTGGGC650.086.963423
ATAGGGC1750.086.135193
TGCAAGG1000.084.839291
AACGGTC452.7284841E-1183.7425463
GAACGGT452.7284841E-1183.7425462
TACGGGG401.0149961E-982.434073
CGGGTAC401.033186E-982.2403645
GGTGCCG401.033186E-982.2403647
CGAAGGG2650.081.767492
Produced by FastQC (version 0.11.5)