FastQCFastQC Report
Mon 27 Feb 2023
SRR3127150.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127150.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences349767
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42211.206803386254278No Hit
GCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTG19710.5635179991251319RNA PCR Primer, Index 14 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTG14440.4128462662286608RNA PCR Primer, Index 16 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGC13440.3842558045784765RNA PCR Primer, Index 16 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCT5780.16525286833806505RNA PCR Primer, Index 16 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCT5620.16067839447403556RNA PCR Primer, Index 16 (95% over 21bp)
GGCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCT5350.1529589698284858RNA PCR Primer, Index 16 (95% over 21bp)
GATCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTC4510.12894298204233104No Hit
ACTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTG4510.12894298204233104RNA PCR Primer, Index 14 (95% over 22bp)
AAAGCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTT4430.1266557451103163No Hit
AGAGCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTT4420.12636984049381447No Hit
GAATCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTT4140.11836451123176286No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT3770.10778604042119469No Hit
GAAGCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTT3670.10492699425617626No Hit
GCAGAGAATATCAACTGTCTCTTATACACATCTGACGCAACCCATGTCGT3630.10378337579016889No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG3570.10206794809115784No Hit
ACCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCT3570.10206794809115784RNA PCR Primer, Index 14 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCC3510.10035252039214677No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACGG201.822E-594.146041
GTACGGG201.832388E-594.038322
GGATGCG156.8987487E-493.9987
ATCGGGC156.8987487E-493.9983
TAGGGCG201.8362956E-593.997994
CGTAGGG950.089.088942
CGTTTTT13200.086.657161
AAGGGTA1400.083.926784
AGGGCAT2300.083.780825
CGAGGGT452.7284841E-1183.553783
AGGGTAC800.082.2482455
GTAGCGG700.080.696621
CAAGGGT1000.079.89833
AGGGCGA1600.079.310815
TACGGGG950.079.156213
TAGTAGG900.078.455041
AGTAGGG3600.078.365272
CGGGTAC301.4542002E-678.331675
GGGTACG301.4542002E-678.331676
AAGGGCA3550.078.1110154
Produced by FastQC (version 0.11.5)