FastQCFastQC Report
Mon 27 Feb 2023
SRR3127147.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127147.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1204288
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136521.1336158792581177No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC78510.6519204708508264No Hit
GCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTG43590.3619566083860339No Hit
CCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTG35930.29835056066323No Hit
CTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC32750.27194491683052563No Hit
AAAGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTT23150.19222976563745547No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC18380.1526212998883988No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCCTC17590.14606140723813574No Hit
GGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCT14610.12131649572195355No Hit
CGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCT14270.11849325078386566No Hit
AGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCT14260.11841021416803953No Hit
AAAGCGGCTGTGCAGACACTGTCTCTTATACACATCTGACGCCTCCAGGT13470.11185032151777648No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGT850.088.4644553
GGGCGAT4350.087.511176
CGTTTTT44600.086.510721
ATAGCGG2150.085.352671
ACGGGTC950.084.0994344
CGCTACG14000.082.244293
AGGGATG31350.081.2511445
TAGCGGG5350.080.857112
GCTACGA14200.080.754964
TCCGCTA14650.079.974721
CGAAGGG10200.079.749892
TAACGGG2600.079.572592
AGGGAAT24950.079.301115
AGGGCGC2200.079.039985
GGATGGC25000.078.766547
GAGGGCG3000.078.32794
GATGACC15350.078.072768
CTACGAC14750.077.743765
AGGGCGA5150.077.567445
AGGGCGT1400.077.208935