FastQCFastQC Report
Mon 27 Feb 2023
SRR3127146.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127146.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3771905
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT504621.3378385722864177No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC64190.170179259551871No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT56300.14926144746487516No Hit
CCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG37990.10071833728580121TruSeq Adapter, Index 14 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT150050.086.0243451
CGTAGGG10000.078.0145342
GAATAGG22350.074.05941
TAGGGCA20300.073.394784
AGGGATG60650.071.680565
TAGGGAT27850.070.5428544
ATAGGGA35000.070.361723
AGTAGGG59200.069.7011952
TAAGGGA34000.069.3901753
ATAGGGC21850.068.6139453
GTAGGGT15700.067.951553
TATAGGG26100.067.5242
ATAGCGG7850.067.0908661
ACGGGAT10900.066.8354954
AGTAAGG22400.066.7565841
GTAGGGA28750.066.36843
TAGGGCG6750.066.148774
ATAAGGG48250.065.844092
TAGCGGG17850.065.821722
TAGAGGG69100.065.768182
Produced by FastQC (version 0.11.5)