FastQCFastQC Report
Mon 27 Feb 2023
SRR3127145.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127145.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2940178
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT377101.2825754086997454No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC48840.16611239183478005No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT44780.15230370406145477No Hit
GCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG36440.12393807449753043TruSeq Adapter, Index 14 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG33630.11438083000416982TruSeq Adapter, Index 14 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC32410.11023142136292428Illumina PCR Primer Index 3 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT116700.086.329341
AGGGATG45650.073.7071155
TAGGGCA15350.073.691884
GAATAGG16700.073.149491
ACGGGAT8100.072.7347264
ACGGGTA2600.072.510924
ATAGGGA26350.072.084383
CGTAGGG6700.071.757012
ATAGGGC18500.071.335073
AGTAAGG16950.071.2357941
TAGGGAT20100.069.877444
TAACGGG9850.069.864352
TATAGGG21350.069.76352
AGTAGGG47700.069.3677752
GTAGGGA20450.068.681493
GAGGGAT36800.068.2646944
GATAGGG41600.067.415952
AGGGCAT26850.067.208495
AGGGAAT33500.066.492215
TAAGGGA28150.066.47053
Produced by FastQC (version 0.11.5)