FastQCFastQC Report
Mon 27 Feb 2023
SRR3127144.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127144.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3153246
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT396261.2566732820718713No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC50020.1586301861637183No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT48010.15225580243342893No Hit
GCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG37570.11914706305819463TruSeq Adapter, Index 14 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG34330.10887193704519088TruSeq Adapter, Index 14 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC31710.10056303884949033Illumina PCR Primer Index 3 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT124450.084.707661
TAGGGCA16750.075.756344
CGTAGGG8550.075.342152
AGGGATG50150.073.0023045
GAATAGG17550.072.939261
ATAGGGC19150.070.434133
AGTAGGG48300.070.092082
AGTAAGG17500.069.9205551
ATAGGGA28850.069.72173
TAGGGAT22400.069.0268254
TATAGGG22000.068.8203352
GTAGGGC14500.068.388723
GTAGGGA24100.068.2528843
GTACGGG6000.068.179142
AGGGCAC24250.068.0245065
ATAAGGG37950.068.021172
ATAGAGG20700.066.841551
TAAGGGA28400.066.358423
AGAGGGC48650.066.269143
AAGGGTC23800.065.756294
Produced by FastQC (version 0.11.5)