FastQCFastQC Report
Mon 27 Feb 2023
SRR3127143.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127143.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2610467
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT259130.9926576355878087No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT70360.2695303177554054No Hit
GCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTG39620.15177360985601426No Hit
CCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTG35440.13576114925030655No Hit
CTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC30940.11852285433985565No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27120.10388945732698403No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT81150.082.097161
CGTAGGG8000.075.196982
TAGGGCG5000.072.379874
ATAGGGC15750.071.914573
TAGGGCA12000.071.6748664
AGGGCAT23300.070.399015
ATAGGGA26500.070.231153
AGGGACT44700.068.13415
ATAAGGG34900.067.87122
GTAAGGG41300.067.822942
AGTAGGG43450.067.820062
ACGGGAT5850.067.487054
TAAGGGA23150.067.198173
GTAGGGC10600.066.950143
AGGGATG36450.066.9216845
GTACGGG6050.066.8072362
ATGAGGG37800.066.767162
ATAGAGG18700.066.124281
AGGGTAT13950.066.035725
CGAAGGG26500.065.797362