FastQCFastQC Report
Mon 27 Feb 2023
SRR3127142.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127142.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2090737
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193650.9262284065379816No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT53440.2556036459870371No Hit
GCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTG38930.1862022817791047No Hit
CCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTG33020.1579347378460323No Hit
CTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC30360.14521195157497094No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT59600.082.463851
CGTAGGG4750.076.363762
ATAGGGC11250.075.787133
TAGGGCA9950.074.32694
GGTAAGG12200.069.910811
AGTAGGG36600.069.889022
GGGCGAT8900.069.172216
AGGGATG26600.069.078915
TAAGGGT8050.069.04873
ATAGCGG3550.069.024071
ATAGAGG13450.068.668791
TAGAGGG37200.068.255252
GTACGGG4500.068.044192
GTAGGGA17250.067.722343
GTAAGGG32550.067.441182
TAGTAGG8250.067.399031
GAATAGG13500.067.018241
ATAGGGA18300.066.925513
AGTAAGG13550.066.770951
AAGGGTA13950.066.521414
Produced by FastQC (version 0.11.5)