FastQCFastQC Report
Mon 27 Feb 2023
SRR3127140.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127140.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3240898
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT767402.367862240650585No Hit
CGTTCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTT57500.1774199619981869No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCT49080.1514395084325394No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT47400.14625575997763582No Hit
CGCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCT40470.12487279760115869No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC39890.12308317015839437No Hit
CCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTG33190.10240988762990999No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT243950.088.666551
AGGGATG56050.073.881475
TAGGGCA16550.072.993424
AGGGTAC22450.072.442765
AAGGGTA27000.071.900994
ATAGCGG7550.071.623191
AGGGCAT27250.071.239155
ATAGGGC20950.070.8943253
AAGGGAT61550.070.7182544
TAGGGTA14700.070.668124
ACGGGTA5500.070.080924
CGTAGGG9200.069.4778062
GAGGGAT48450.069.368274
AGGGACT45850.069.198975
ATAGGGA31950.068.993853
TAGAGGG62250.068.857382
GAATAGG19850.068.6973951
GTAGGGT11950.068.4368063
GTAGGGA24550.067.9653
AGGGTAT19650.067.934595
Produced by FastQC (version 0.11.5)