FastQCFastQC Report
Mon 27 Feb 2023
SRR3127139.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127139.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2575321
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT581512.2580097781985238No Hit
CGTTCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTT53370.20723630180470706No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCT46010.1786573401917664No Hit
CGCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCT40610.15768908031270665No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT36660.14235118651228335No Hit
CCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTG30810.11963557164330194No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC29840.11586905088724862No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT184450.089.165711
ATAGGGC17050.076.013113
GAATAGG15850.074.967221
GAGGGAT34850.073.5658654
AGGGATG40900.073.5352555
TAGGGCA13000.072.5048144
AGGGTAC18000.072.0568855
AAGGGAT45850.071.6429754
TAGGGTA10150.071.5047454
GTAGGGT10750.071.020993
ATAGCGG5450.070.079111
ACTAGGG13200.069.979492
AGGGATC21450.069.887955
CGTAGGG7700.069.775472
ACGGGTA4200.069.570094
AAGGGTA20750.069.4997944
AGGGAAT29500.069.454985
AGGGCAT20850.069.419835
GTAGGGA19250.069.039653
AGGGACT34800.068.869965
Produced by FastQC (version 0.11.5)