FastQCFastQC Report
Mon 27 Feb 2023
SRR3127138.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127138.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2673958
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT599672.2426305873166297No Hit
CGTTCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTT51860.1939447066857445No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCT44230.1654102270865885No Hit
CGCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCT40590.15179744782827553No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT38880.14540243339648565No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC31150.11649397634517819No Hit
CCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTG29370.1098371776968823No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT192950.089.145951
ACGGGTA5050.075.381984
AAGGGTA22500.073.3159564
AGGGCAT21000.072.510295
AGGGATG39700.072.094265
GTAGGGT9800.071.9348143
CGTAGGG7750.071.590812
GTACGGG7400.070.529082
TAGGGCA11800.070.496114
AGTAAGG18000.069.806811
AAGGGAT46600.069.7901464
ATAGGGA25550.069.714363
TAGGGTA11800.069.699554
AGGGACT35500.069.6356055
AGGGTAC18100.069.5873265
ATAGCGG5750.069.568091
GAATAGG17000.069.483851
AGGGAAT30600.069.4210055
AGAGGGC36350.069.041593
ATAGGGC17100.068.98453
Produced by FastQC (version 0.11.5)