FastQCFastQC Report
Mon 27 Feb 2023
SRR3127137.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127137.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3142902
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT493701.570841216175369No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC62930.20022896036847476No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC33750.1073848309619581No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTT32060.10200763498193707No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT169050.086.963291
ATAGCGG5300.081.6084751
AGGGATG92250.081.366265
AAGGGAT71050.075.148454
GGATGGC75450.074.3167957
CGGGATG18650.073.336465
GAGGGAT61650.072.959984
TAAGGGA35650.072.772053
AGGGAAT49250.071.479485
ACGGGAT12450.070.973184
GATGACC57900.070.6230558
CGTAGGG9450.070.621132
GGAATGC40900.070.443837
ATAGGGC18450.070.052143
GGGATGA69700.069.118916
ATAGGGA36700.068.897153
TAGGGCA18850.068.818244
TAGCGGG16000.068.734382
ATGGCAC73300.068.417359
CGAAGGG30950.068.3329242
Produced by FastQC (version 0.11.5)