FastQCFastQC Report
Mon 27 Feb 2023
SRR3127136.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127136.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2506144
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT372981.488262446212189No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC49720.1983924307621589No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTT30140.12026443811688395No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC26500.10574013304901872No Hit
CGCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCT25960.10358542845103874No Hit
CCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTG25890.10330611489204132No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT125100.087.67171
AGGGATG71150.080.578755
ACGGGTA3550.074.3661354
CGTAGGG6300.074.0815662
AAGGGAT54000.073.420574
GGATGGC61100.073.297197
GAATAGG15000.073.275441
ACGGGAT10400.072.9807054
ATAGCGG4450.072.084631
CGGGATG13900.072.011275
TAAGGGA23900.071.40463
ATAGGGA29500.071.2735443
CGAGGGA15150.070.94763
GAGGGAT50250.070.737684
GATGACC44850.070.620878
AGAGGGC36250.070.486643
AGGGAAT38600.069.881185
AGTAGGG39700.069.3486252
TAGGGAT25700.068.971594
AGGGCAT23100.068.964145
Produced by FastQC (version 0.11.5)