FastQCFastQC Report
Mon 27 Feb 2023
SRR3127135.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127135.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2619136
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT385991.4737302683022189No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC51160.19533159026488126No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC29780.11370161763268498No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTT29330.11198349379337308No Hit
CGCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCT26890.10266744453132637No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT130250.087.594441
AGGGATG74600.080.891095
CGTAGGG8300.079.32132
ATAGCGG5200.077.84251
ACGGGAT11350.077.431954
CGGGATG15700.074.537395
GGATGGC60400.073.297387
AAGGGAT56750.073.042764
GACCGAT2100.071.615198
ATGCGGG12150.071.6036762
AGAGGGC39450.071.479043
GGAATGC32600.071.361197
TAGGGAT26350.070.9866944
GATGACC48600.070.979718
GTAGGGT10550.070.830313
GAGGGAT49550.070.7570344
CGCTACG10600.070.496213
ATAGGGC15000.070.182893
TAAGGGA24700.070.020523
AAGGGCG11700.069.491994
Produced by FastQC (version 0.11.5)