FastQCFastQC Report
Mon 27 Feb 2023
SRR3127134.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127134.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2954694
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT285450.9660898895114012No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT66780.22601325213372347No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC55990.18949508815464478No Hit
CTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC49360.16705621631207834Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTG47180.1596781257213099No Hit
CCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTG36670.12410760640526566No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT91350.082.240081
ACGGGAT8200.075.664914
TAGGGCA16800.074.422944
ATAGGGA27450.071.914983
AGGGATG48650.071.8802955
GTAGGGT9300.071.765853
CTACGAC10550.071.728745
ATAGGGC17500.071.173813
GAGGGAT43400.070.614244
AGGGAAT38600.070.138255
CGTAGGG7600.069.882742
TAGGGTC7900.069.613464
AGTAGGG45800.069.269712
TAGGGAT20300.069.000894
GTACGGG7800.068.090872
AGAGGGA75250.068.082013
AGTACGG3600.067.907261
AGGGTAC11450.067.732675
TAGAGGG53900.067.6671752
AAGGGAT50500.067.015554
Produced by FastQC (version 0.11.5)