FastQCFastQC Report
Mon 27 Feb 2023
SRR3127133.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127133.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2343329
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206860.8827612341246149No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT53100.22660070352903927No Hit
CTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC49510.21128061829986314Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTG44120.18827915329004163No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC41200.17581824831255022No Hit
CCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTG34030.14522075218631272No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT64600.082.046561
ACGGGAT6850.079.8056644
CGTAGGG6850.076.370672
GTAGGGT7850.074.4420553
TAGGGCA12800.074.3717044
AGTAGGG37200.073.861782
ATAGGGA21200.073.3574753
AGGGATG36400.072.5564965
AGGGAAT29800.072.38325
AGTTAGG8200.071.3073651
TAGGGTC5950.071.2839664
ATAGGGC14900.070.848073
TAGCGGG11050.070.80112
TAAGGGA22850.070.329013
GTAGGGC11900.070.0958943
TAGGGCG3850.069.771884
GGTAAGG13950.069.633721
GAGGGAT32950.068.937884
AGGGTAC9000.068.4022145
AGGGTAA9350.068.3547445
Produced by FastQC (version 0.11.5)