FastQCFastQC Report
Mon 27 Feb 2023
SRR3127132.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127132.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2447001
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT216160.8833670276391388No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT57760.23604403921371506No Hit
CTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC51950.21230068970139368Illumina Single End Adapter 1 (95% over 21bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC43830.17911721327453484No Hit
GCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTG43230.17666523225777186No Hit
CCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTG33530.13702487248677056No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT69150.082.132311
TAGGGCG4250.075.197594
ACGGGAT6500.074.474544
TAGGGCA14350.074.3460544
GTAGGGT8500.074.091753
TAGGGAC13400.071.900694
AGTAGGG39500.071.895442
GGGCGAT10000.070.9677356
CTACGAC9550.070.8668445
AGGGAAT29850.070.8520055
GAATAGG15950.070.802661
ATAGGGA21800.070.713343
CGTAGGG5800.070.5265662
GTAGGGA19500.069.413173
GTAGGGC13800.069.1354753
GTACGGG4900.069.087252
GACCGAT2800.068.819248
TAGGGTC5950.068.72054
ATAGGGC15550.068.608743
GAGGGAT33550.068.501534
Produced by FastQC (version 0.11.5)