FastQCFastQC Report
Mon 27 Feb 2023
SRR3127130.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127130.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2663865
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT259920.9757251212054665No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT57570.21611455535471957No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC50350.18901107976567882No Hit
GCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTG29920.11231800410306078No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT83700.085.793311
AGGGATG58450.076.139035
AGGGAAT33200.073.038795
AGTAGGG42800.072.341582
TAGCGGG16650.072.175922
CGTAGGG8200.070.689952
GAATAGG16400.070.149561
ATAGGGA24800.069.928523
AGGGCAT25100.069.8356255
CGAAGGG27200.069.650382
ATAGGGC15100.069.59633
AAGGGAT55600.069.07824
GAGGGAT36800.069.023854
TAGGGCA13800.068.981164
TAAGGGA25900.068.960073
GTAGGGA24300.068.846253
AGGGTAC9900.068.829515
TAGGGTA8150.068.8093644
GGTAAGG16800.067.918021
GTACGGG5850.067.669012
Produced by FastQC (version 0.11.5)