FastQCFastQC Report
Mon 27 Feb 2023
SRR3127129.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127129.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2770300
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT267680.9662491426921271No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT61780.22300833844709958No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC53570.1933725589286359No Hit
GCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTG28440.10266036169367938No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT85700.085.4502261
AGGGATG60550.075.7531055
TAGGGCA16250.075.483484
GAGGGAT37350.073.483024
AGGGTAC12000.072.061185
AAGGGTA19350.071.891164
CGAAGGG27950.071.324212
AGGGAAT32550.071.180445
GGTAAGG18450.070.403971
ATGCGGG14150.069.7775652
ATAGGGC18400.069.728383
AGTAGGG46600.069.617132
GTACGGG6150.069.569552
AGGGATA30400.069.567075
AAGGGAT57100.069.136594
TAAGGGA26600.069.08123
GTAGGGA22200.068.377673
ATAGGGA27200.068.2484743
AGAGGGC38500.067.992223
TAGCGGG15800.067.847012
Produced by FastQC (version 0.11.5)