FastQCFastQC Report
Mon 27 Feb 2023
SRR3127128.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127128.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3455686
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT318850.9226822112888728No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT84140.24348277013594408No Hit
GCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTG59790.17301919213724856No Hit
CCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTG54200.15684295390264044No Hit
CTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC52880.15302316240538058No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC37960.10984794336059468No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT101850.083.089911
ATAGGGC18700.074.1423653
AGTAGGG58150.072.8206942
TAGGGCA17050.071.946294
GTAGGGC16500.071.779863
CGTAGGG10500.069.825652
GAATAGG21650.069.708221
AGGGATG53500.069.488835
GTAGGGA30050.069.129563
AGTACGG4450.068.673671
GGAGCTA69050.068.4060759
TAGAGGG61000.068.1086352
ATGCGGG17350.067.178762
ATAGGGA34550.066.927273
ACTAGGG16200.066.43552
TAAGGGA30400.066.3238453
TGAAGGG60000.066.18892
TGTAGGG23750.065.8961942
TAGCGGG14900.065.6082
AAGAGGG116450.065.3411942
Produced by FastQC (version 0.11.5)