FastQCFastQC Report
Mon 27 Feb 2023
SRR3127127.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127127.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2706471
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT231950.8570200826094202No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT65610.2424190024574437No Hit
GCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTG57060.21082804877643246No Hit
CCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTG45960.16981523171687413No Hit
CTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC44470.16430990762509556No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC28500.10530317893670392No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT75950.082.844051
GTAGGGC12850.076.321793
TAGGGCA13600.074.193054
AGTAGGG42700.072.331242
TAAGGGA23000.070.931083
AGGGAAT31750.070.4525765
CGTAGGG8900.069.9352952
TAGACGG4000.069.5705261
CGAAGGG26600.069.489132
TAGCGGG11350.069.379812
GTAGGGA22300.069.351723
AGGGATG42300.068.98985
ATAGGGC15550.068.527733
AGAGGGC37700.068.037113
ATGCGGG11300.068.0176542
GGTAAGG18350.067.600971
GAGGGAT33600.067.49854
ATAGCGG4850.067.10281
TACGGGT3000.066.011013
AGAAGGG95150.065.5637052
Produced by FastQC (version 0.11.5)