FastQCFastQC Report
Mon 27 Feb 2023
SRR3127125.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127125.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2960762
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT277970.9388461483901779No Hit
GCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTG42670.14411830467967368No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT40900.13814011393013015No Hit
CCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTG39680.13401955307451258No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC38460.129898992218895No Hit
CTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC36550.12344795022362486Illumina Single End Adapter 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT84550.081.865931
AGGGATG54000.076.681935
TAGGGCA19600.074.099164
CGTAGGG7900.073.1722
ATAGCGG5550.072.864241
ACGGGAT9350.070.376444
ATAGGGC20250.070.323293
TAGAGGG48750.070.085262
AGTAGGG53400.069.79092
GTAGGGC17500.068.75173
AAGGGTA20250.068.703294
AGGGTAC13900.068.642355
AAGAGGG101850.068.383932
TAAGGGA29650.068.1593253
AGGGAAT33850.068.037495
GTAGGGA26750.067.993713
AAGGGAT58600.067.9354864
ATAAGGG42200.067.933542
GAGGGAT42650.067.66444
AGGGCAT27750.067.411035
Produced by FastQC (version 0.11.5)