FastQCFastQC Report
Mon 27 Feb 2023
SRR3127123.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127123.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2466791
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207260.840200892576631No Hit
GCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTG35790.14508728141135588No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT34040.13799304440465365No Hit
CCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTG32530.13187173133029917No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC32130.13025019144305294No Hit
CTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC29000.11756164182535123Illumina Single End Adapter 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT67450.082.9463961
AGGGATG42250.075.752735
ATAGGGC16750.073.7936253
TAGGGCA14950.073.561844
TATAGGG21250.073.458492
CGTAGGG7200.073.1388852
ATAGCGG6200.072.0990451
AGTAGGG43450.070.662052
TAAGGGA23700.070.595923
GTAGGGA23700.070.397613
ACGGGAT7300.070.174864
ATAGGGA24400.069.726313
AGGGAAT29700.069.626435
TAGAGGG42950.069.2952352
GAGGGAT35350.069.1340264
AGGGCAT23350.068.6349645
TACGGGT1850.068.591443
GTAGGGC13650.068.172693
TAGACGG3400.067.813281
AGAGGGC35500.067.782813
Produced by FastQC (version 0.11.5)