FastQCFastQC Report
Mon 27 Feb 2023
SRR3127122.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127122.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3607714
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT893902.4777462958538288No Hit
CGTTCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTT75500.20927379498485743No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCT66150.18335710646686518No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT60380.16736359922100255No Hit
CGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCT57630.15974104377453424No Hit
CCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG48610.13473906191011814No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT283300.087.4365161
ATAGGGC21600.077.244863
TAGGGCA19750.071.8708654
ATAGCGG9500.070.284321
AGTAGGG54800.068.7832642
ATAGGGA33500.067.904023
AGAGGGC55000.067.765013
AGGGATG56350.067.0590065
ACGGGAT10350.066.755964
TAGCGGG19950.066.434722
GTACGGG8600.066.126552
ATAGAGG25550.065.884881
TATAGGG27700.065.493242
TAGAGGG66750.064.918522
GGTAAGG23500.064.6291351
AGGGACT47150.064.593425
GAATAGG20700.064.285021
AGATAGG21200.064.099651
AGTAAGG21800.064.060991
TAAGGGA31200.063.8713723
Produced by FastQC (version 0.11.5)