FastQCFastQC Report
Mon 27 Feb 2023
SRR3127120.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127120.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3003969
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT694902.3132728733219285No Hit
CGTTCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTT67560.22490245405328751No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCT59630.19850404581405467No Hit
CGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCT53980.1796955960597463No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT49220.1638498932578865No Hit
CCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG38710.12886284778571283No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT224550.087.559711
ATAGGGC18150.073.5392463
TAGGGCA14650.072.501724
AGTAGGG43700.071.545782
AGGGATG46950.070.7719655
AGAGGGC47600.068.81823
ATAGCGG8250.067.880021
TAAGGGA27500.067.335023
ATAGGGA25900.067.321163
ATAGAGG20500.067.26091
GTAGGGT10850.066.2733843
AGGGACT38150.066.0309755
GGTAAGG20350.065.6754151
GTAGGGA21750.065.040633
AGTAAGG20350.064.981661
AGATAGG18550.064.43751
GAGGGAT35350.064.3477554
GTAAGGG51000.064.254922
GAATAGG18400.064.195541
AAGAGGG98600.064.18182
Produced by FastQC (version 0.11.5)