FastQCFastQC Report
Mon 27 Feb 2023
SRR3127119.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127119.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences614588
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69851.1365337429302231No Hit
GCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTG19370.31517048819697097No Hit
CCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTG15680.2551302661295047No Hit
CTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC14170.23056096116422709Illumina Single End Adapter 2 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCT6740.10966696388474881No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC6650.1082025682245667No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT25600.084.490491
TACGGGA1750.083.253323
CGAAGGG4300.079.787032
AGTACGG1250.078.994941
AGGGTAC1800.078.336115
CGAGGGA4400.076.905563
CTACGAC1050.076.097945
AATACGG255.5451474E-575.233281
GTACGGG1500.075.1965562
ACGGGAT2100.073.8597644
GAGGGAT8800.073.707164
TTACGGG1150.073.5618442
GGGTACG452.6284397E-973.11376
AGGGAAT10250.071.99285
AAGGGAC6750.071.721074
GGGCGAT2100.071.621596
AGGGCAC4050.070.792635
CGGGTAC200.002162195370.50255
GGCGATA800.070.50257
TAGCGGG2350.069.996792
Produced by FastQC (version 0.11.5)