FastQCFastQC Report
Mon 27 Feb 2023
SRR3127117.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127117.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences512014
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53411.0431355392625983No Hit
GCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTG17090.33377993570488307No Hit
CCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTG13660.26678957997242264No Hit
CTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC11740.2292906053350104Illumina Single End Adapter 2 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCT5970.11659837426320374No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC5360.10468463752944254No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGGG1050.089.535622
CGAAGGG3550.086.067692
CGTTTTT17600.085.799951
ATAACGG850.083.016991
CGCTACG1050.080.566323
GCGGGTA1000.079.894944
ACGGGAA1550.078.833714
AGGGCGT1200.078.328375
GAGGGAT6200.078.075694
GATGCGG551.8189894E-1276.979391
ACGGGAT1850.076.211394
CGGGTAT507.094059E-1175.195245
CGGGTAA507.094059E-1175.195245
CACGGGC255.558179E-575.195243
TAGCGGG1700.074.6569062
AAGGGAC5250.074.300054
TCACGGG700.073.866892
CAGGGTA1150.073.5605554
GGTAAGG3050.072.492431
TTAGCGG650.072.373791
Produced by FastQC (version 0.11.5)