FastQCFastQC Report
Mon 27 Feb 2023
SRR3127116.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127116.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2346747
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT256581.093343253448284No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT66040.2814108210216099No Hit
GCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTG56210.23952305041830244No Hit
CCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTG40800.1738576847014186No Hit
CTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC39040.1663579414397888No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT78500.084.033871
ATAGGGC15250.073.0398253
CGTAGGG6450.072.862322
TAGGGCA12100.072.2482454
AGTACGG2900.071.338011
ATGCGGG11700.070.695142
ATAGGGA22800.070.49733
TAGGGAC14050.069.9148944
GACCGAT1550.069.7407468
TAGAGGG40200.069.442142
ATAAGGG31750.069.273142
AGGGCAT17950.069.125645
AGTAGGG33100.068.8614962
ATGAGGG34800.068.333552
ATAGGGT11900.068.325113
ACGGGAT5250.068.038394
GTAGGGA19250.067.628583
AGGGAAT27800.067.626195
GTACGGG4600.067.429322
GAGGGAT32000.067.122164
Produced by FastQC (version 0.11.5)