FastQCFastQC Report
Mon 27 Feb 2023
SRR3127115.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127115.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1891760
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189791.0032456548399376No Hit
GCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTG57140.302046771260625No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT51530.2723918467458874No Hit
CCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTG39470.2086416881634034No Hit
CTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC38700.2045714044064786No Hit
AGCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCT21210.11211781621347316No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT58700.085.5963061
TAGGGCA9300.078.5931854
CGTAGGG4300.076.767482
AGGGAAT21600.072.229425
AGTAGGG28050.072.122722
ATGCGGG8350.071.724072
GGTAAGG10800.071.204491
ATAGGGC11350.071.045473
ATGAGGG28800.070.5722
ATACGCG200.002164540370.488969
TAGAGGG30050.069.6764762
AAGGGTA14300.069.5797044
ATAAGGG24800.069.40472
GTACGGG3550.069.075292
CGAGGGA10650.069.073453
ACGGGAT5550.068.8221364
ATAGGGA17200.068.8147353
GTAGGGC8600.068.540573
GTAGGGT7300.068.472973
GCAGGGA39500.068.047773
Produced by FastQC (version 0.11.5)