FastQCFastQC Report
Mon 27 Feb 2023
SRR3127114.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127114.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1993748
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197580.9909978593081974No Hit
GCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTG57580.28880279754512606No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT56440.28308492347076963No Hit
CTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC40200.20163029630625334No Hit
CCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTG39790.19957386791109008No Hit
AGCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCT20390.10226969506677876No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT60950.082.922821
CGTAGGG6350.077.01322
TAGGGCA10800.076.15184
TAGCGGG8250.074.6662
ATAGGGC14350.074.342963
AGGGAAT21850.072.9145365
GTAGGGC9100.071.785923
AGGGCAT16700.071.198355
AGTAGGG28450.070.7403262
ATAGGGA19150.069.9426353
AGGGCGA9450.069.6245045
AGAGGGC25500.068.743963
GGGCGAT9250.068.0814746
ATAGAGG11200.068.067611
AAGGGTA15550.068.001434
ATGCGGG10100.067.973092
AGGGTAC8450.067.852995
ACGGGAT5750.067.8384864
ATGAGGG29400.067.8147352
AGGGCTC16650.067.742755
Produced by FastQC (version 0.11.5)