FastQCFastQC Report
Mon 27 Feb 2023
SRR3127113.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127113.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3407441
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT345371.0135758770291254No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT60590.17781672521989375No Hit
GCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTG39250.11518908177720465TruSeq Adapter, Index 16 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT104300.082.48861
CGTAGGG10000.073.789222
AGTAGGG54200.073.447582
GGTAAGG19350.072.161021
ATAGGGA31350.070.761623
TAGAGGG64300.070.60892
TATAGGG29150.069.330322
ACTAGGG18600.068.730472
TAGGGCA15700.068.5554354
TAGGGTA12450.068.3306054
ATAGGGC19550.068.0350953
AGAGGGC48550.067.861283
ATGAGGG56000.067.142162
AAGGGAC36150.066.828374
AGGGATG48600.066.5352555
TAGACGG6550.066.034931
AGGGCAT31750.066.022325
AGGGACT49250.065.943425
GAATAGG21750.065.927671
ACGGGAT9100.065.594644
Produced by FastQC (version 0.11.5)