FastQCFastQC Report
Mon 27 Feb 2023
SRR3127112.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127112.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2752528
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT257110.934086774049165No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT47530.1726776258043515No Hit
GCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTG39530.14361343463172765TruSeq Adapter, Index 16 (100% over 22bp)
CCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTG32580.11836391855051065TruSeq Adapter, Index 16 (100% over 22bp)
CTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC30130.10946301000389459TruSeq Adapter, Index 16 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT80100.083.434531
CGTAGGG8650.075.204182
AGTAGGG44500.073.515412
TAGAGGG49600.071.0884252
ATAGGGC17050.071.051283
GAATAGG16900.070.885191
GTAGGGA22350.070.652973
ATAGGGA21750.069.56793
GGTAAGG16050.068.762141
TATAGGG21900.068.448192
TAGGGAC14100.068.19844
ATAAGGG34450.068.1426542
AGGGATG36750.067.90055
AAGGGGC82200.067.3797154
AGAGGGC39700.067.32183
AGGGAAT31100.067.241195
TAGGGCA12000.067.170414
AGGGACT41650.067.020435
ATAGCGG5300.066.741191
GTACGGG6600.066.422922
Produced by FastQC (version 0.11.5)