FastQCFastQC Report
Mon 27 Feb 2023
SRR3127111.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127111.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2856852
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT266000.9310947854491587No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT48900.1711674248438491No Hit
GCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTG38230.1338186227357945TruSeq Adapter, Index 16 (100% over 22bp)
CCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTG31100.10886108205815354TruSeq Adapter, Index 16 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT82000.081.9549941
CGTAGGG7000.074.5547262
GGTAAGG16350.073.110041
ATAGGGC18200.072.044463
TAGGGCA13650.071.958394
GAATAGG17600.071.9284441
AGTAGGG47300.071.270242
TAGACGG3950.070.2936251
ATAGGGA25350.070.077993
ACGGGAT7000.069.823744
TATAGGG22300.069.365142
AGAGGGC40500.069.044593
ATAAGGG39450.068.4092942
AATAGGG41500.067.635772
TAGAGGG49600.067.396622
GTACGGG6750.066.86792
AAGGGGC82700.066.716064
AGGGCAT26500.066.6821755
AGTAAGG18850.066.659331
GTAGGGA22100.066.348353
Produced by FastQC (version 0.11.5)