FastQCFastQC Report
Mon 27 Feb 2023
SRR3127110.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127110.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2537639
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT272601.0742268699369768No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT67590.2663499418159951No Hit
GCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTG35590.14024847505890317No Hit
CTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC35010.1379628859739309No Hit
CCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTG32420.12775654850827875No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26850.10580701195087244No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT78250.082.036671
ACGGGAT7100.081.426684
AGGGATG35600.071.557015
AGTAGGG40250.069.826632
ATAGGGA23100.068.97213
ATGAGGG38550.068.638812
TATAGGG20150.068.340532
CGAAGGG23050.067.694422
ATAGGGC15700.067.654173
GTAGGGA19050.067.599163
GAGGGAT28000.067.482014
AAGAGGG84800.067.28352
TAGGGTC8500.066.909294
AGGGCAT20350.066.74775
TAGGGCA12700.066.617564
TAACGGG8050.066.557152
TAAGGGA20800.065.7529753
TAGCGGG11800.065.7185062
AGGGAAT28650.065.620315
TAGTAGG11350.065.052421
Produced by FastQC (version 0.11.5)