FastQCFastQC Report
Mon 27 Feb 2023
SRR3127109.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127109.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1959152
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200641.0241165565509975No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT51230.2614906857660866No Hit
CTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC36240.1849779904775127No Hit
GCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTG35250.1799247837840045No Hit
CCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTG32130.1639995263256756No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT59200.084.056751
AGGGCAT14500.075.1885455
AGGGATG27150.074.426965
TAGGGTC7350.074.406744
AGTAGGG30300.071.574252
ATAGGGA16600.071.57053
ATAGGGC10950.071.041393
AGGGTAT10100.070.256635
GTAGGGT9750.070.114043
ATAGAGG14200.070.0867541
TAGCGGG10200.069.331842
TAGGGCA9050.068.764934
TAGGGTA6800.068.638524
ACGGGAT5450.068.339564
AGGGAAT22150.067.253865
TATAGGG13000.067.091892
TAGGGCG3750.066.632524
AGAGGGC29600.066.577313
TAGAGGG32200.066.4724352
ATAAGGG25350.066.394472
Produced by FastQC (version 0.11.5)