FastQCFastQC Report
Mon 27 Feb 2023
SRR3127108.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127108.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1993912
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205501.0306372598188887No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT53730.2694702674942525No Hit
CTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC36870.18491287479086338No Hit
GCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTG34390.1724750139424408No Hit
CCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTG31140.15617539791124183No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT64000.083.7696151
AGGGATG26050.073.6070565
AGTAGGG32350.070.790062
TAAGGGA16000.070.4949953
TAGGGCA8750.069.8236164
AGGGCAT15550.069.512755
GTAGGGT9400.069.495073
ATAGGGA17250.069.200893
AAGGGTA12800.069.026354
ATAGGGC13150.068.2613143
TAGTAGG8900.068.224831
AGTAAGG10700.068.185291
TAGGGTC6550.068.16314
ACGGGAT5000.067.67524
AGGGTAT10700.067.2008365
AAGGGCG10650.066.6337664
GTAGGGA15300.066.3482363
TAGAGGG32400.066.326762
TAGCGGG8300.066.286542
AGGGAAT21100.066.15175
Produced by FastQC (version 0.11.5)