FastQCFastQC Report
Mon 27 Feb 2023
SRR3127106.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127106.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences811304
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104351.286200980150474No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC61240.7548341928549595No Hit
GCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTG34440.4245017897113782No Hit
CCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTG30200.3722402453334385No Hit
CTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC25590.3154181416583673No Hit
AAAGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTT13820.1703430526658318No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCTCC13130.1618382258684784No Hit
AGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCT10860.13385857828877953No Hit
CGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCT10630.13102363602299508No Hit
GGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCT10620.13090037766361315No Hit
TAGACCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCT8530.1051393805527891No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGCGA156.904445E-493.989839
CGTTTTT29800.088.5691451
CGCTACG10400.086.505233
CGAAGGG5200.084.24072
CTACGAC10800.083.111395
GCTACGA10850.082.917464
TACGGGT401.0168151E-982.429063
TACGACC10850.082.295246
CGTAGGG1800.081.120672
ACGGGAT4300.081.0598144
ACGACCA10800.080.935697
AGGGATG22650.080.9183965
GGATGGC13650.080.9077
ACGGGTC353.805326E-880.746844
AGGGAAT15100.080.607175
TCCGCTA11250.080.437651
CGAGGGA4400.080.288053
CGACCAA11150.079.238068
TAACGGG2200.079.2175452
ATGGCAC12850.078.26399
Produced by FastQC (version 0.11.5)