FastQCFastQC Report
Mon 27 Feb 2023
SRR3127105.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127105.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences831363
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105161.2649107549890963No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC63430.7629639519680332No Hit
GCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTG32860.3952545398339835No Hit
CCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTG28690.34509594485200806No Hit
CTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC26280.31610740434683765No Hit
AAAGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTT13650.16418820659567482No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCTCC12770.15360317935727233No Hit
CGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCT10350.12449435445166551No Hit
AGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCT10200.12269008844511965No Hit
GGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCT10120.1217278132416285No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGT201.8382618E-593.993143
GATTCGA156.9035386E-493.993148
CGTAGGG2400.088.160992
CGCTACG12600.085.78743
CGTTTTT31150.085.688621
GCTACGA12750.084.409524
TACGGGA1850.083.8317263
CGGGTAT900.083.549465
CTACGAC12900.083.428025
TACGACC12850.083.386926
AGGGATG18550.083.352415
TCCGCTA13200.082.382741
CGAGGGA4850.081.3961263
TAGCGGG3950.080.9444352
ACGACCA13150.080.7697757
GCGTAGG1050.080.701451
AATGCGG1700.080.3058551
CGACCAA13250.079.8055048
AGGGTAC2600.079.532665
CTAGCGG950.079.285641