FastQCFastQC Report
Mon 27 Feb 2023
SRR3127104.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127104.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1613699
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218031.3511193847179679No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC115160.7136399043439947No Hit
GCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTG61660.3821034777861299TruSeq Adapter, Index 22 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC57140.35409329744890466TruSeq Adapter, Index 20 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTG56860.3523581535342093TruSeq Adapter, Index 22 (95% over 21bp)
AAAGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTT31020.1922291579780368No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC24720.15318841989739104No Hit
AGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCT21400.13261457062314597No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCTCT20750.1285865579640317No Hit
ACTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTG17010.10540999281774359No Hit
TAGACCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCT16960.10510014569011941No Hit
CGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCT16780.10398469603067238No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT63000.087.458971
AGGGATG43800.081.774415
TAGGGCA8550.080.264464
CGCTACG18450.079.73413
CGTAGGG4200.079.449612
TAGGGCG1850.078.763774
CGAAGGG10950.078.5452042
ACGGGAT7550.078.444074
GGATGGC35250.078.404557
TCCGCTA19050.078.231491
AGGGCAT13400.078.223385
ACGGGTA2050.077.958164
AGGGTAC6550.077.503085
AGGGAAT29800.077.446495
CGGGATG8450.076.764235
CGGGAAT5150.076.667025
GCGGGAT8350.076.557714
AATGCGG2900.076.193361
AGGGTAT11450.075.535115
GAATGCC18350.075.0506748
Produced by FastQC (version 0.11.5)