FastQCFastQC Report
Mon 27 Feb 2023
SRR3127103.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127103.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1317095
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154571.1735675862409316No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC87740.666163033038619No Hit
GCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTG57050.4331502283434377TruSeq Adapter, Index 22 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTG49150.37316974098299666TruSeq Adapter, Index 22 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC45690.34689980601247444TruSeq Adapter, Index 20 (95% over 21bp)
AAAGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTT28340.2151705078221389No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC19410.14736977970457713No Hit
AGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCT19380.14714200570194255No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCTCT18970.1440290943326032No Hit
TAGACCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCT15550.1180628580322604No Hit
CGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCT15120.11479809732783132No Hit
GGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCT14920.1132796039769341No Hit
AGAGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTT14160.10750932924352458No Hit
ACTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTG13200.10022056115921783No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT46000.086.699071
ACGGGAT5400.084.618594
CGGGAAT4000.084.5902565
AGGGATG35100.082.474845
CGTAGGG3150.082.250762
CGCTACG16800.081.87693
GTACGGG2600.081.531792
TAGGGCA6950.081.336274
TCCGCTA17200.080.0306551
GCTACGA17250.079.740984
CGGGATG6900.079.686485
AGGGTAC4900.079.603085
GGATGGC28550.079.339377
AGGGAAT24600.078.1332865
CGAGGGA8600.077.781753
GAATGCC15300.077.710018
CGAAGGG9200.077.3173452
GATGACC20750.077.003188
GGAATGC16650.076.489697
ACGACCA17100.076.400567
Produced by FastQC (version 0.11.5)