FastQCFastQC Report
Mon 27 Feb 2023
SRR3127102.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127102.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1365603
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159051.164686955139964No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC92710.6788942320718393No Hit
GCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTG55620.407292602608518TruSeq Adapter, Index 22 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTG47980.3513466212361865TruSeq Adapter, Index 22 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC45060.3299641257378609TruSeq Adapter, Index 20 (95% over 21bp)
AAAGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTT27840.203865984477187No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC20240.14821291400209285No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCTCT19490.14272083467889277No Hit
AGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCT18790.13759489397723937No Hit
CGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCT15700.11496752716565503No Hit
GGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCT15140.11086677460433231No Hit
AGAGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTT14990.10976835873969229No Hit
TAGACCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCT14050.10288495265461485No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT47800.088.0098651
AGGGATG36550.085.381045
AGTACGG2100.085.150271
AGGGTAC6400.082.981115
ACGGGTA1700.082.937914
ATAGCGG2300.081.837791
GGATGGC31550.081.781267
CGTAGGG4150.081.580782
CGCTACG17800.081.058623
CGGGAAT4050.080.070925
TAGGGCA7750.080.048464
ACGGGAT6600.079.042344
GTACGGG3700.078.7940442
TCCGCTA18650.078.469941
AGGGAAT24750.077.855535
ATGGCAC30050.077.730729
CGGGATG8000.077.546955
CGAAGGG9650.077.477082
GAGGGAT23200.077.1823044
ACGGGTG1950.077.1251754
Produced by FastQC (version 0.11.5)