FastQCFastQC Report
Mon 27 Feb 2023
SRR3127101.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127101.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3452108
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT409951.1875352683056266No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC102720.2975573186006927No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC37260.10793405073074192No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT144000.086.75831
ATAGCGG6800.082.976361
ACGGGAT22500.081.470534
AGGGATG123900.080.916665
GCGGGAT22200.078.760484
CGGGATG22600.078.6143345
TAGGGCA29850.078.5732354
AAGGGAT112950.077.317524
CGAGGGA27550.076.087413
CGAAGGG30650.074.674592
ATAGGGC29550.074.595893
GATGACC54000.074.245038
GAGGGAT87900.073.845364
TAGGGAT45900.073.524184
AAGGGCG19950.073.507234
TAGGGCG8700.072.3942264
GTAGGGC21950.072.3738563
AGGGATC40750.072.204655
ATAGGGA53750.072.139813
AGGGAAT66750.071.9644555
Produced by FastQC (version 0.11.5)