FastQCFastQC Report
Mon 27 Feb 2023
SRR3127100.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127100.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2709101
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT309601.1428145351539127No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC77180.2848915562764179No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC29230.10789557126146276No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT115100.087.1461
ACGGGAT15850.085.6496354
AGGGATG95550.081.59315
CGGGATG17700.079.6499945
TAGGGCA23050.078.527654
AAGGGAT86500.078.416414
TAGCGGG14000.077.439422
GCGGGAT17900.076.367274
TAGGGAT37400.076.251094
ATAGCGG5650.075.953581
GGATGGC64850.075.435977
CGAGGGA22700.074.9624253
CGAAGGG22850.074.676622
CGTAGGG7200.074.633652
GAGGGAT67400.074.06254
AGGGAAT48800.074.053275
AGGGATT55400.073.119845
TAAGGGA37600.072.586023
AGAGGGC49700.072.365323
AGAGGGA96000.072.27683
Produced by FastQC (version 0.11.5)