FastQCFastQC Report
Mon 27 Feb 2023
SRR3127098.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127098.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1656831
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162530.9809690909935895No Hit
GCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG55560.3353389693939816Illumina PCR Primer Index 7 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG51570.3112568511815629Illumina PCR Primer Index 7 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC50080.30226377946815336TruSeq Adapter, Index 23 (96% over 25bp)
AGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCT19490.11763420650627614TruSeq Adapter, Index 23 (95% over 23bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC18440.11129680697669225No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGGG4400.087.590622
ATAGCGG2250.085.667441
CGTTTTT60700.085.505651
AGTACGG1650.082.628241
TAGGGCA13500.079.38954
CGAAGGG12200.076.663682
TCGAGGG5700.075.034852
AGGGACT21750.074.5582355
TAAGGGA19700.074.436323
AGGGATC15850.074.4355245
TAAGGGC16050.074.379823
TAGCGGG7800.074.115152
TAACGGG3500.073.856912
AAGGGAC17400.073.7522664
ACGGGAT5750.073.576084
AGGGCAT20850.073.2677845
AAGGGCG8100.073.121914
AGGGTAG8850.072.763425
AAGGGCA34600.072.684144
AAGGGAT35750.072.581354
Produced by FastQC (version 0.11.5)