SRR3127097.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3127097.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1345503
Sequences flagged as poor quality	0
Sequence length	100
%GC	42

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11374	0.8453344214022563	No Hit
GCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG	5178	0.38483749200113265	Illumina PCR Primer Index 7 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG	4511	0.3352649529581131	Illumina PCR Primer Index 7 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC	4134	0.3072456917598846	TruSeq Adapter, Index 23 (96% over 25bp)
AGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCT	1834	0.1363059019563687	TruSeq Adapter, Index 23 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCT	1467	0.10902985723554685	TruSeq Adapter, Index 23 (95% over 23bp)
AAAGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTT	1450	0.10776638922395565	TruSeq Adapter, Index 23 (95% over 21bp)
GGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCT	1434	0.10657724286010511	Illumina PCR Primer Index 7 (95% over 21bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC	1347	0.10011125950666777	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
ATAGCGG	300	86.38258	1
CGTTTTT	4325	85.19329	1
AGGGATG	2300	77.43993	5
TAGGGCA	980	77.378944	4
CGAAGGG	1060	77.31834	2
TAACGGG	330	77.07596	2
AAGGGAT	2950	75.83934	4
AGGGCAC	1080	75.71449	5
TAGGGCG	270	75.01147	4
TAAGCGG	190	74.39648	1
AGGGAAT	2085	73.70471	5
ATAGGGC	1055	73.66386	3
GACCGAT	180	73.10365	8
CGAGGGA	710	72.972176	3
AGGGATC	1370	72.72251	5
AGGGCAT	1605	72.322845	5
TAAGGGA	1420	72.30879	3
GTAGGGC	785	72.000336	3
AGAGGGC	1800	71.95868	3
TAGCGGG	675	71.87413	2