SRR3127096.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3127096.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1404318
Sequences flagged as poor quality	0
Sequence length	100
%GC	42

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11927	0.8493090596289445	No Hit
GCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG	5048	0.3594627427690879	Illumina PCR Primer Index 7 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG	4434	0.31574045194891753	Illumina PCR Primer Index 7 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC	3976	0.28312675619054944	TruSeq Adapter, Index 23 (96% over 25bp)
AGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCT	1807	0.12867455946587597	TruSeq Adapter, Index 23 (95% over 23bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC	1493	0.10631495145686377	No Hit
CGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCT	1446	0.10296813114978232	TruSeq Adapter, Index 23 (95% over 23bp)
GGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCT	1406	0.1001197734416279	Illumina PCR Primer Index 7 (95% over 21bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	4505	85.7499	1
ATAGCGG	240	84.30289	1
CGTAGGG	200	82.27783	2
AGTACGG	100	79.98972	1
GTAGGGC	855	78.05153	3
CGAGGGA	895	77.18866	3
CGAAGGG	985	75.416374	2
AGGGATG	2415	75.31007	5
TAGGGCA	1030	74.828285	4
AGGGCAT	1565	74.772896	5
AGGGTAT	945	74.09922	5
AGGGCAC	1245	73.98538	5
TAAGGGA	1460	73.390724	3
AAGGGCA	2490	72.85295	4
AAGGGTA	1200	72.84351	4
AGGGAAT	2175	72.81651	5
TCGAGGG	550	72.66095	2
TAGGGTA	420	71.61267	4
AAGGGAT	2790	71.08328	4
GTACGGG	225	71.04625	2