FastQCFastQC Report
Mon 27 Feb 2023
SRR3127094.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127094.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences868507
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101101.1640666108620885No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC50690.5836452671078068No Hit
GCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTG36120.41588611260473435No Hit
CCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTG29130.3354031688863763No Hit
CTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC23720.27311236409148115No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC14170.1631535497123224No Hit
AAAGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTT13130.15117897725637214No Hit
GGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCT11440.13172029701545296No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCACA10510.12101226587695896No Hit
AGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCT10450.12032142515834646No Hit
CGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCT10030.11548554012805884No Hit
AGAGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTT9380.10800143234308993No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA9200.10592891018725238No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGGT600.086.41383
GTAGGGT2300.086.072243
CGTTTTT29350.085.8125461
ATAACGG500.084.896571
CGGGTAT1400.083.917315
CGCTACG9600.082.976893
ATTACGG401.0077201E-982.538321
TCCGCTA9800.082.29771
CGAAGGG7000.082.149222
CTACGAC9800.081.999215
ACGGGTA1150.081.9735644
ATAGCGG1500.081.752251
AGGGATG22700.081.566155
CGTAGGG1850.081.5304642
ACGGGAT4050.081.467564
TAGGGTA2550.081.3306354
AGGGAAT18650.081.1365665
GTACGGG2000.080.1291662
ATAGAGG5350.079.342591
ACGACCA10200.078.7835547
Produced by FastQC (version 0.11.5)